We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to\ndifferent cryopreservation lengths (2ââ?¬â??1159 days) from patients of varying ages (26ââ?¬â??62 years). Subcutaneous abdominal adipose\ntissue was excised during abdominoplasties and was cryopreserved.The viability and number of adipose-derived stem cells isolated\nwere measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation\nduration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue\ncryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were\nno significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient\nage. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently,\nlonger cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with\ncontinued cell growth. Patient age does not significantly impact stemcell isolation, viability, or growth. Cryopreservation of adipose\ntissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages.
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